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Mobility of Membrane Lipids and Proteins at the Animal and Vegetal Pole of the Sea Urchin Egg
Author(s) -
PETERS R.,
SARDET C.,
RICHTER H.P.
Publication year - 1984
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1984.00105.x
Subject(s) - sea urchin , paracentrotus lividus , fluorescence recovery after photobleaching , photobleaching , fluorescence , membrane , biophysics , polarity (international relations) , biology , chemistry , biochemistry , microbiology and biotechnology , optics , physics , cell
We have compared the mobility of a fluorescent lipid analogue and of fluorescently labeled membrane proteins at the animal and vegetal poles of the egg of the sea urchin Paracentrotus lividus. Translational diffusion coefficients have been measured by fluorescence microphotolysis (“photo‐bleaching”) on the egg which was rotated on its poles. Lipid and protein diffusion coefficients averaged 0.8 μm 2 /sec and 0.04 μm 2 /sec, respectively at both animal and vegetal pole of the egg. Substances which were known to animalize (Zn ++ ) or vegetalize (Li + ) the sea urchin egg had no significant effect on protein diffusion coefficients.