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Respiration of Sea Urchin Spermatozoa in the Presence of a Synthetic Jelly Coat Peptide and Ionophores
Author(s) -
SUZUKI NORIO,
OHIZUMI YASUSHI,
YASUMASU IKUO,
ISAKA SABRO
Publication year - 1984
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1984.00017.x
Subject(s) - coat , sea urchin , peptide , chemistry , respiration , biochemistry , biology , chromatography , botany , microbiology and biotechnology , ecology
Sperm respiration and motility of the sea urchin Hemicentrotus pulcherrimus were studied at pH 6.8 in the presence of a synthetic jelly peptide (Gly‐Phe‐Asp‐Leu‐Asn‐Gly‐Gly‐Gly‐Val‐Gly) and monovalent cationic ionophores. The synthetic peptide stimulated sperm respiration and motility to the level of that found in normal sea water (pH 8.2) with half‐maximal stimulation at approximately 100 pM. Monensin and valinomycin also stimulated sperm respiration with half‐maximal effects at 7 μM and 0.7 μM, respectively. The stimulation of sperm respiration by the peptide and monensin was dependent on external Na + , but was not dependent on the osmolarity of the suspending medium. Approximately 50 mM Na + was required for half‐maximal respiratory responses to the peptide and monensin.