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The Inhibitory Effect of 5‐Bromodeoxyuridine on the Programmed Cell Death in the Chick Limb
Author(s) -
TONÉ SHIGENOBU,
TANAKA SHOJI,
KATO YOSHIHIRO
Publication year - 1983
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1983.00381.x
Subject(s) - mesenchyme , bromodeoxyuridine , programmed cell death , staining , inhibitory postsynaptic potential , microbiology and biotechnology , biology , cell , limb bud , thymidine , anatomy , immunohistochemistry , immunology , endocrinology , biochemistry , apoptosis , dna , genetics , mesenchymal stem cell , embryo
The inhibitory effect of 5‐bromodeoxyuridine (BrdU) on the programmed cell death in interdigital mesenchyme of a chick leg bud was studied using vital staining and histological methods. A single administration of BrdU at day 6 1/3 specifically inhibited the programmed cell death in the mesenchyme of interdigital areas of a leg bud, resulting in the formation of a web‐like structure. No inhibitory effect was observed on general development of limb buds. Hoechst 33258 staining revealed that many cells of interdigits incorporated BrdU into their nuclei. The simultaneous administration of thymidine blocked the BrdU effect.