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Changes in Activities of Glucose Metabolising Enzymes in Germ Cells during Spermatogenesis in Rats
Author(s) -
NAKAMURA MASAHISA,
FUJIWARA AKIKO,
YASUMASU IKUO,
OKINAGA SHOICHI,
ARAI KIYOSHI
Publication year - 1983
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1983.00281.x
Subject(s) - pentose phosphate pathway , dehydrogenase , spermatogenesis , glucose 6 phosphate dehydrogenase , enzyme , biology , fructose , glycolysis , endocrinology , medicine , biochemistry , chemistry
The rate of 14 CO 2 , liberation from [ 14 C‐1]glucose was identical to that from [ 14 C‐6]glucose in spermatids, but more than the latter in spermatogonia. Rotenone (1 μM) completely inhibited 14 CO 2 release from [ 14 C‐1]glucose in spermatids, but decreased it only 30% in spermatogonia. The activity of glucose‐6‐phosphate dehydrogenase, but not 6‐phosphogluconate dehydrogenase, was markedly lower in spermatocytes and spermatids than in spermatogonia. The activities of the glycolytic enzymes, glucosephosphate isomerase, fructose diphosphatase, glyceraldehyde‐3‐phosphate dehydrogenase and enolase, differed only slightly in spermatids and spermatogonia. It is concluded that the low glucose‐6‐phosphate dehydrogenase activity may contribute to the low activity of the pentose cycle in spermatocytes and spermatids.