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Influence of Temperature on Glucose Metabolism of Round Spermatids of Rats
Author(s) -
NAKAMURA MASAHISA,
FUJIWARA AKIKO,
SUZUKI AKIRA,
OKINAGA SHOICHI,
ARAI KIYOSHI
Publication year - 1983
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1983.00049.x
Subject(s) - aldolase a , dihydroxyacetone phosphate , glycolysis , glyceraldehyde , fructose , fructose bisphosphate aldolase , medicine , metabolism , anaerobic glycolysis , enzyme , endocrinology , carbohydrate metabolism , chemistry , fructolysis , biochemistry , biology , dehydrogenase
Glucose utilization by spermatids was found to be 17.37±0.37 nmoles/hr/10 6 cells at 34°C and 28.94±1.12nmoles/hr/10 6 cells at 40°C. A good parallelism was observed between the increased rate of glucose utilization and lactate production at 40°C. There was no significant change in the levels of glycolytic intermediates in the cells, except for marked accumulations of fructose‐1, 6‐diphosphate, dihydroxyacetone phosphate and glyceraldehyde‐3‐phosphate in the presence of glucose (1 mM). Glucose oxidation in the citrate cycle by spermatids was higher at 40°C than at 34°C, but was never greater than 2% of the overall rate of glucose utilization. In addition, glucose did not prevent decrease of ATP at either 34 or 40°C. The effects of temperature on the activities of 11 glycolytic enzymes were examined. The activities of aldolase and phosphoglyceromutase were similar between 30 and 34°C, but increased markedly at 40°C. The higher temperature increased the V max values, without affecting the Kms. The activities of other glycolytic enzymes were similar at the different temperatures. These findings indicate that the increased overall rate of glucose utilization in glycolysis at higher temperature is due to increased Vmax values of aldolase and phosphoglyceromutase.