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Novel Inducers and Inhibitors of Differentiation of Friend Erythroleukemia Cells: Application of an Opal Glass Transmission Method to Study of Erythroid Differentiation
Author(s) -
MORIOKA KIYOKAZU,
TANAKA KIYOKO,
ISHIZAWA MINORU,
ONO TETSUO
Publication year - 1982
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1982.00507.x
Subject(s) - cellular differentiation , chemistry , inducer , biochemistry , hemoglobin , microbiology and biotechnology , biology , gene
The potencies of poly(ADP‐ribosylation)‐inhibitors in inducing erythroid differentiation of Friend erythroleukemia cells were surveyed. Picolinamide and m‐aminobenzamide were newly found to be inducers, whereas compounds related caffeine did not induce differentiation. In other series of experiments some bile acids suspected of being tumor promoters were found to inhibit the differentiation like typical tumor promoters such as phorbol esters. These modifications of erythroid differentiation were detected by an opal glass transmission method. This method is simpler than any previously reported methods, and is sufficiently reliable to use in determining hemoglobin in living cells as a quantitative marker of erythroid differentiation.