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Abnormal Limb Regeneration without Tumor Production in Adult Newts Directed by Carcinogens, 20‐Methylcholanthrene and Benzo (α) pyrene
Author(s) -
TSONIS PANAGIOTIS A.,
EGUCHI GORO
Publication year - 1982
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1982.00183.x
Subject(s) - blastema , carcinogen , regeneration (biology) , biology , pyrene , anatomy , benzo(a)pyrene , microbiology and biotechnology , genetics , astrobiology
The effects of potent carcinogens, 20‐methylcholanthrene (MC) and benzo(α)pyrene (BP), on limb regeneration were studied in adult newts. A microcrystal of these carcinogens was administered directly to the blastema of forelmibs on day 7 after amputation. The formation of the regeneration cone was delayed and the cone was shifted in abnormal polarity depending upon the site of micro‐crystal administration. These carcinogens affected morphogenesis of skeletal structures of regenerating limbs. Subregeneration and superregeneration of either or both carpals and digits, absence of either or both ulna and radius, and accessory limb formation have been recorded as abnormalities caused by these carcinogens. Non‐carcinogenic benzocompounds did not show such effects as those of MC and BP. The regeneration blastema of the limb appears to be resistant to carcinogenic effects of the carcinogens used since tumor formation has never been observed in our study so far.