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Inhibition of Respiration in Sea Urchin Spermatozoa following Interaction with Fixed Unfertilized Eggs VI. Probable Difference between the Species in the Mechanism for the Fixed‐Egg‐Induced Inhibition of Sperm Respiration
Author(s) -
FUJIWARA AKIKO,
HINO AKIYA,
HIRUMA TAKAAKI,
YASUMASU IKUO
Publication year - 1982
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1982.00145.x
Subject(s) - hemicentrotus , respiration , biology , sea urchin , sperm , sperm motility , biochemistry , anatomy , botany , ecology
In spermatozoa of all examined sea urchins, the respiration was inhibited and their motility was lowered by the glutaraldehyde‐fixed eggs. The respiration of the fixed‐egg‐reacted spermatozoa was stimulated by 2, 4 dinitrophenol in Clypeaster japonicus, Hemicentrotus pulcherrimus and pseudocentrotus depressus but was not in Anthocidaris crassispina and Toxopneustes pileolus. Ratio of ADP to ATP was markedly lower in the reacted spermatozoa of the former species than in those of Anthocidaris. The low respiratory rate in the former species probably results from ADP control but does not in the latter species. Tetramethyl‐p‐phenylenediamine enhanced the respiratory rate in the reacted spermatozoa of the latter species to almost the same rate as in the intact spermatozoa, but elevated slightly in the former species. The inhibition of electron transport in mitochondrial respiratory chain is probably predominant in the latter species. In the former species, the slight inhibition of electron transport does not seem to result in a failure of ADP phosphorylation, and hence the stop of movement probably causes a shortage of ADP. Carnitine, which made the reacted spermatozoa of all species motile, enhanced the respiratory rate only in those of the former species.