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Evidence of a Neural Inductive Phase in Limb Development
Author(s) -
VARDY P. H.,
STOKES P. A.,
McBRIDE W. G.
Publication year - 1982
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1982.00099.x
Subject(s) - neural tube , neural crest , embryo , limb development , anatomy , somite , neural plate , biology , embryogenesis , nervous system , mesoderm , neural fold , dorsum , limb bud , neural development , neuroscience , microbiology and biotechnology , biochemistry , embryonic stem cell , gene
To demonstrate a causal relationship between neuronal damage and peripheral mesodermal anomalies, an operation was performed through oblique incisions in the dorsum of 247 chick embryos at stages 12–15 of development. In the region of the 24–30th presumptive somites, a three to four somite length of the right half of the neural tube was cut with fine glass sytlettes and removed with a 40μm glass pipette. Immediately after the operation and at intervals during continued incubation (37±, 75% R.H.), embryos were processed for histology. Fifty one embryos survived until day 14 when they were examined for malformations. Excision frequently resulted in a variety of reduction deformities: some embryos appeared normal; 31 displayed unilateral hemimelia or amelia. Examination of serially sectioned embryos fixed immediately and at varying intervals after the operation, demonstrated that this range of malformations was consistent with variation in the amount of neural tissue excised. Disruption of the mesoderm and regeneration of nervous tissue was minimal. It is concluded that the nervous system per se or some factor produced by the nervous system is essential for the development of the limb.