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Partial Purification of the Sperm‐binding Factor from the Egg of the Sea Urchin, Anthocidaris Crassispina , Followed by an Immunological Method
Author(s) -
YOSHIDA MOTONOBU,
AKETA KENJI
Publication year - 1982
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1982.00055.x
Subject(s) - sea urchin , sperm , human fertilization , sodium dodecyl sulfate , biology , polyacrylamide gel electrophoresis , antibody , gel electrophoresis , chemistry , precipitin , biochemistry , anatomy , microbiology and biotechnology , botany , immunology , enzyme
Univalent antibody (Fab fragments) against sperm‐binding factor inhibits the fertilization of eggs species‐specifically. The sperm‐binding factor was partially purified from unfertilized eggs of the sea urchin Anthocidaris crassispina by monitoring its neutralizing effect on fertilization inhibiting Fab fragments. It formed two species‐specific precipitin lines by the double‐immunodiffusion test and gave three main bands of protein with apparent molecular weights of 80,000, 87,000 and 225,000, on sodium dodecyl sulfate‐polyacrylamide gel electrophoresis. Carbohydrate was detected in the first and third of these protein bands.