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Different Growth Responses of Hamster Dermal Fibroblasts and Chondrocytes to Platelet–derived Growth Facter in Cell Culture
Author(s) -
KATOH Y.,
TAKAYAMA S.
Publication year - 1981
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1981.00523.x
Subject(s) - platelet derived growth factor , platelet , growth factor , hamster , platelet derived growth factor receptor , cell culture , thymidine , chemistry , fibroblast , microbiology and biotechnology , dna synthesis , chinese hamster , andrology , endocrinology , medicine , immunology , biology , dna , biochemistry , genetics , receptor
The growth responses of dermal fibroblasts and chondrocytes obtained from 1‐week‐old hamsters to a growth factor from platelets [platelet‐derived growth factor (PDGF)] were compared. Autoradiography showed that most of the whole nuclei of dermal fibroblasts were labeled with 3 H‐thymidine in 5% whole blood serum (WBS), which contains platelet releasate, but only a few percent were labeled in 5% plasma‐derived serum (PDS), which is free of platelet releasate. Addition of platelet releasate to PDS restored the growth stimulatory activity of the serum for dermal fibroblasts. In contrast, most of the nuclei of chondrocytes were labeled with 3 H‐thymidine both in 5% WBS and 5% PDS. Dermal fibroblasts grew in 5% WBS, but not in 5% PDS, whereas chondrocytes grew in both 5% WBS and 5% PDS. Partially purified PDGF added to the medium with 0.5% fetal calf serum stimulated DNA synthesis in dermal fibroblasts, but not in chondrocytes.