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Maintenance and Growth of the Horny Processes of the Medaka, Oryzias latipes, in Vitro
Author(s) -
IWATA AKIKO,
UWA HIROSHI
Publication year - 1981
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1981.00245.x
Subject(s) - oryzias , in vitro , androgen , biology , organ culture , secretion , andrology , microbiology and biotechnology , endocrinology , fish <actinopterygii> , medicine , biochemistry , fishery , hormone
The maintenance and growth of the horny processes of the medaka, Oryzias latipes , were investigated in vitro by organ culture of the anal‐fin. Processes were induced in adult females by oral administration of ethisterone. On this day, anal‐fins were removed and cultured with in media containing androgen. Early processes elongated in these media during six to eight days of culture, though they degenerated in androgen‐free control media. They developed to nearly the same degree as those in intact fins in medium 199 and CMRL‐1066, while they degenerated to various extents in Eagle's MEM and Dulbecco's modified EM. These results suggest that scleroblasts maintain the mass and secrete the horny substance in vitro in the presence of androgen and essential nutrients. Early processes developed on the fifth day of treatment.