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INDUCING TIME FOR NEURAL TISSUES IN GASTRULA ECTODERM OF CYNOPS PYRRHOGASTER
Author(s) -
OHARA AKITO
Publication year - 1981
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1981.00051.x
Subject(s) - ectoderm , gastrulation , anatomy , biology , embryo , microbiology and biotechnology , embryogenesis
The inducing times for spinal cord and deuterencephalon in Cynops gastrula were determined by the sandwich method. The extreme posterior of the archenteron roof at the slit‐blastopore stage (tail organizer) was used as an inducer. First, the presumptive ectoderm of the earliest gastrula (0‐hr stage) was put in contact with the organizer for 6 to 24 hr. Spinal cord and deuterencephalon were induced in almost all explants after 24 and 21 hr of contact, respectively, indicating that 24 hr is enough time for differentiations of both spinal cord and deuterencephalon. Next, presumptive ectoderm of 6‐ to 21‐hr exogastrulae was put in contact with the organizer until the 24‐hr stage. Results showed that the net inducing times for spinal cord and deuterencephalon were 18 and 15 hr, respectively, and that neural competence appeared in the presumptive ectoderm at the 6‐hr stage (straight‐blastopore stage).