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STRAIN‐SPECIFIC APPEARANCE OF A POSSIBLE FETAL α‐GLOBIN POLYPEPTIDE CHAIN IN MICE
Author(s) -
IKAWA YOJI,
SOMA GENICHIRO,
MATSUGI TAKAYASU,
OBARA NAOKO,
SUDO KATSUKO,
SUZUKI KIYOSHI,
MORIWAKI KAZUO
Publication year - 1981
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1981.00001.x
Subject(s) - yolk sac , globin , fetus , embryo , biology , microbiology and biotechnology , chemistry , gene , biochemistry , genetics , pregnancy
DDD mouse embryos at 12–16 days of gestation have a putative ‘fetal’α‐globin polypeptide chain, detected by its lower electrophoretic mobility than the authentic α‐globin chain on acidic urea‐Triton gel. When hepatic erythroid cells of DDD embryos were contaminated with yolk sac erythroid cells, two α‐bands were visible, but only ‘fetal’α seemed to be synthesized, because in embryos of strain ddY which share a common ancestry with DDD, only ‘fetal’α was detectable by autoradiography when the hepatic erythroid cells of embryos of more than 15 days of gestation showed an authentic adult α‐globin chain other than this ‘fetal’α‐globin chain. If this ‘fetal’α‐globin chain is structurally different from the yolk sac α and adult peripheral blood α‐globin chains, then switching over of the transcription of α‐globin polypeptide chain occurs in hepatic erythroid cells of mid‐late DDD embryos. However, it is still possible that this ‘fetal’α‐globin chain is formed by post‐translational modification of the authentic α‐globin chain. The α‐globin chains of hepatic erythroid cells of C57BL/6 and BALB/c embryos had similar mobilities to those of yolk sac and adult erythroid cells.