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POLYOL ACCUMULATION IN IN VITRO SYSTEMS OF BOMBYX EGGS
Author(s) -
KAGEYAMA TAKASHI,
TAKAHASHI SUSUMU Y.,
OHOKA TADAKAZU,
OHNISHI EIJI
Publication year - 1980
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1980.00859.x
Subject(s) - sorbitol , polyol , glycerol , incubation , trehalose , bombyx mori , in vitro , chemistry , bombyx , bombycidae , biochemistry , food science , biology , organic chemistry , polyurethane , gene
The mechanism of polyol accumulation in diapausing Bombyx eggs, conversion of [6‐ 14 C] glucose‐6‐phosphate into polyols and other neutral sugars was investigated in in vitro reaction systems. When a crude homogenate or a press juice of the eggs was incubated with [6‐ 14 C]glucose‐6‐P, the labelled trehalose, sorbitol and glycerol accumulated in the reaction mixture. In the press juice incubation system of developing eggs at day 1, 14 C‐sorbitol was detected in appreciable amounts, but it decreased rapidly with the development of the embryos. When the press juice was prepared from eggs in diapause, the formation of 14 C‐sorbitol was 3–5 times greater in eggs at early stages (day 2 to day 4) than in developing eggs.