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BEHAVIOR OF SECONDARY SPERMATOGONIA OF THE NEWT, CYNOPS PYRRHOGASTER , IN IN VITRO CULTURE
Author(s) -
ABE SHINICHI,
TANAKA SHIGEYASU
Publication year - 1980
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1980.00851.x
Subject(s) - mitosis , organelle , sertoli cell , microbiology and biotechnology , biology , in vitro , germ cell , electron microscope , cell , cell type , nucleus , anatomy , spermatogenesis , endocrinology , genetics , physics , gene , optics
Several cell types migrated cut from small pieces of newt testes cultivated in vitro. Flat fibroblastic cells migrated out within a few days. Then, secondary spermatogonia, identified by the presence of germ cell‐specific substances and by the shape and appearance of their nucleus and subcellular organelles, migrated out over the sheet of fibroblastic cells. Sertoli cells co‐migrated with secondary spermatogonia, maintaining a similar cellular arrangement to that of testicular cells in vivo. Mitosis of secondary spermatogonia both in clusters and as single cells was frequent from the third day until about 2 weeks after inoculation. During mitosis, active and periodic rotation of chromosomes was observed. Identification of the cell types and studies on their behavior were performed by electron microscopy and phase contrast microscopy.