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INHIBITION OF RESPIRATION IN SEA URCHIN SPERMATOZOA FOLLOWING INTERACTION WITH FIXED UNFERTILIZED EGGS
Author(s) -
YASUMASU IKUO,
FUJIWARA AKIKO,
HINO AKIYA
Publication year - 1980
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1980.00429.x
Subject(s) - respiration , sperm , sea urchin , biology , glutaraldehyde , andrology , anatomy , zoology , botany , chemistry , ecology , chromatography , medicine
Glutaradehyde‐fixed unfertilized eggs of the sea urchin, Pseudocentrotus depressus , which bound spermatozoa, were capable of causing an inhibition of respiration in 2.3 ± 0.27 × 10 4 spermatozoa per egg. After stirring a suspension containing fixed eggs (2 × 10 3 ‐2 × 10 4 per ml) and a larger number of spematozoa than 2.5 × 10 4 per egg for 15 min at 20°c, the fixed eggs isolated from the suspension failed to cause any inhibition of respiration of fresh spermatozoa, and were incapable of binding fresh eggs. Fixed eggs, treated with a smaller number of spermatozoa than 2 × 10 4 per egg, had a capacity to bind spermatozoa and inhibited sperm respiration. Unless the fixed eggs lost the sperm‐binding capacity, they exerted an inhibitory effect on the respiration of spermatozoa.