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INHIBITORY EFFECT OF RABBIT SERUM FACTOR ON IN VITRO OOCYTE MATURATION OF THE TELEOST FISH, ORYZIAS LATIPES *
Author(s) -
IWAMATSU TAKASHI,
TAKAMA KOJI
Publication year - 1980
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1980.00229.x
Subject(s) - oryzias , oocyte , inhibitory postsynaptic potential , in vitro , chemistry , biology , endocrinology , medicine , gonadotropin , andrology , biochemistry , microbiology and biotechnology , fish <actinopterygii> , embryo , hormone , fishery
The present study indicates that a factor in rabbit serum inhibits the in vitro steroid‐ and gonadotropin‐induced maturation of oocytes of the teleost fish, Oryzias latipes. Such inhibitory activity could not be recognized in the serum of this fish or in the fluids from mammalian follicles. Passage of the serum inhibitor through a cellulose membrane indicated that it has a molecular weight of less than 3,500. The inhibitory activity on steroid‐induced oocyte maturation was not destroyed by heating, by repeated freezing and thawing or by treatment with proteolytic enzymes, lipase or amylases. However, its activity could be removed by extraction with charcoal but not with ethyl ether or toluene. The inhibitory action of the heat‐stable and dialyzable serum factor was reversible. The factor appears to exert its inhibitory effect upon the oocyte itself in an early step of maturation, rather than upon the follicle cells.