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CHANGES IN CELL SURFACE MORPHOLOGY DURING DIFFERENTIATION OF MICROMERE‐ AND MESOMERE‐DERIVED CELLS OF THE SEA URCHIN EMBRYO
Author(s) -
SANO KIYOSHI,
USUI NORIKO
Publication year - 1980
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1980.00179.x
Subject(s) - blastula , blastomere , biology , sea urchin , embryo , polarity in embryogenesis , microbiology and biotechnology , cell , anatomy , embryogenesis , gastrulation , genetics
Micromeres and mesomeres isolated from 16‐cell embryos of the sea urchin, Strongylocentrotus intermedius , were cultured in vitro , and changes in the cells surface architecture during the differentiation of the micromere‐ and mesomere‐derived cells were observed using scanning electron microscopy. Two types of the distribution of the surface microvilli were observed in both blastomere‐derived cell masses. One type showed a uniform distribution of the microvilli and the other type showed an uneven one. Though many microvilli were observed in most of both mesomere and micromere‐derived cells at the 64‐cell stage and the early blastula stage (16 hr after the 16‐cell stage at 6°C) respectively, the microvilli decreased in number at the later stages in both blastomere‐derived cell masses as compared with the 64‐cell stage and the early blastula stage respectively. Rapid disappearance of the surface microvilli was observed in the micromere‐derived cells in contrast with the mesomere‐derived cells which still had many microvilli even at the midmesenchyme stage.