Premium
5S RNA AND TRANSFER RNA SYNTHESIS IN GERMINAL VESICLE OOCYTES AND DURING HORMONALLY‐INDUCED MEIOTIC MATURATION OF STARFISH OOCYTES
Author(s) -
O'MELIA ANNE F.
Publication year - 1979
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1979.00493.x
Subject(s) - germinal vesicle , rna , transfer rna , nucleolus , biology , starfish , microbiology and biotechnology , meiosis , ribosomal rna , oocyte , chemistry , biochemistry , gene , embryo , ecology , cytoplasm
The incorporation of 3 H‐guanosine as 3 H‐GMP into 5S RNA and into transfer RNA (tRNA) was examined in isolated large germinal vesicle oocytes, in isolated mature ootids and during and subsequent to hormonally (l‐methyladenine)‐induced meiotic maturation in the starfish, Asterias forbesii .Purified soluble RNA 1 preparations at each stage were fractionated by electrophoresis on 10% polyacrylamide gels, while high molecular weight RNAs were resolved by subjecting total RNA samples to electrophoresis on 2.4% acrylamide+0.5% agarose gels. The results showed that large germinal vesicle oocytes, containing a single compact nucleolus, synthesize 5S RNA and tRNA as well as the previously‐reported (1, 23‐26) nucleolar rRNAs. In contrast, during and subsequent to hormonally‐induced meiotic maturation, after germinal vesicle braekdown and nucleolar dissolution, the synthesis of 5S RNA and tRNA continues in the absence of detectable high molecular weight rRNA synthesis.