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EFFECTS OF THE LENS CAPSULE ON CELLULAR FLATTENING, CELL GROWTH AND EXPRESSION OF THE DIFFERENTIATED TRAITS OF CHONDROCYTES CULTURED IN VITRO
Author(s) -
TSUNEMATSU YASUHIKO
Publication year - 1979
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1979.00437.x
Subject(s) - capsule , lens (geology) , pseudopodia , microbiology and biotechnology , flattening , in vitro , cellular differentiation , anatomy , biology , cell , chemistry , materials science , biochemistry , botany , actin , paleontology , gene , composite material
The morphology, growth and differentiation of chondrocytes cultured on the lens capsule were studied. When incubated in Eagle's MEM with fetal serum, chondrocytes on the surface of the lens capsule became flattened with extended pseudopodia, but most cell remained spherical on the surface of a plastic dish. Thus, the lens capsule promoted cellular flattening of chondrocytes. When grown in Ham's FI2 medium, flattening of cells on the lens capsule was greater and the cells developed the features of fibroblasts without any detectable characteristics of chondrocytes, although their growth rate was not enhanced. This inhibitory effect of the lens capsule on differentiation in this medium was reversed when the cells were separated from the lens capsule and grown on a plastic substrate.