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DIFFERENTIATION OF RAT LENS EPITHELIAL CELLS IN TISSUE CULTURE (III) FUNCTIONS IN VITRO OF A TRANSFORMED RAT LENS EPITHELIAL CELL LINE
Author(s) -
MILLER G. G.,
BLAIR D. G.,
HUNTER E.,
MOUSA G. Y.,
TREVITHICK J. R.
Publication year - 1979
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1979.00019.x
Subject(s) - rous sarcoma virus , cell culture , lens (geology) , microbiology and biotechnology , biology , embryo , in vitro , epithelium , cell , cellular differentiation , virology , virus , genetics , gene , paleontology
Epithelial cells isolated from one‐day‐old rat lens were neoplastically transformed using Rous Sarcoma Virus (Schmidt‐Ruppin‐D strain) and have been in continuous culture for over 30 months. This cell line displays some of the characteristics of lens epithelial cells, but can be shown to represent a permanent cell line in that it has been transferred more than 50 times and is continuing to grow, while primary lens cell cultures have never survived beyond 8 weeks. The infecting viral genome can be rescued from the cell line upon fusion with uninfected chick embryo cells. It is anticipated that this newly established cell line, designated RLE‐R, will be useful in studies on cellular differentiation.