Premium
BINDING OF CONCANAVALIN A TO THE SURFACE OF STARFISH FOLLICLE CELLS AND PRODUCTION OF 1‐METHYLADENINE
Author(s) -
KUBOTA JUNKO,
KANATANI HARUO
Publication year - 1978
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1978.00349.x
Subject(s) - starfish , concanavalin a , follicle , chemistry , inducer , microbiology and biotechnology , oocyte , endocrinology , biology , andrology , biophysics , biochemistry , in vitro , embryo , ecology , medicine , gene
Starfish follicle cells, treated with concanavalin A (Con A), continued to produce 1‐methyl‐adenine (1‐MeAde), an inducer of starfish oocyte maturation, after rinsing with artificial seawater (ASW). On the other hand, they ceased to produce the substance if treated with methyl α‐Dmannoside (αMM). These cells produced again 1‐MeAde when re‐stimulated with Con A after removal of αMM. An optical study with fluorescein revealed that Con A bound to the cells was not dissociated by rinsing with ASW, but was removed if the cells were treated with αMM. These results suggest that continuous binding of Con A to the surface of the follicle cells is essential for the production of 1‐MeAde.