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CHANGE IN THE ACTIVITY OF ARYLESTERASE IN SEA URCHIN EGGS FOLLOWING FERTILIZATION
Author(s) -
MITA M.,
YASUMASU I.
Publication year - 1978
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1978.00219.x
Subject(s) - sea urchin , human fertilization , arylesterase , guanosine , centrifugation , biology , microsome , chemistry , enzyme assay , enzyme , zoology , biochemistry , anatomy , ecology , pon1 , gene , genotype
The activity of arylcsterase in sea urchin eggs ( Anthocidaris craxsispina ), increases at 5 min after fertilization to about 1.5‐fold that in unfertilized eggs, and decreases at 15 min to a lower level than that in unfertilized eggs. Then the activity of the enzyme increases again. The enzyme activity in unfertilized eggs is enhanced by either fructose 1, 6‐diphosphate (FDP) at concentrations between 4 and 10 μM, or guanosine 3′, 5′‐cyclic monophosphalc (cGMP) at concentrations between 0.1 and 0.3 μM. The activity is detectable in the crude microsomal fraction and also in the supernatant fraction obtained from sea urchin egg homogenates by centrifugation at 105,000 × g for 2 hr.