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VEGETALIZATION OF SEA URCHIN LARVAE INDUCED WITH cAMP PHOSPHODIESTERASE INHIBITORS
Author(s) -
YOSHIMI TAKAHITO,
YASUMASU IKUO
Publication year - 1978
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1978.00213.x
Subject(s) - blastula , theophylline , sea urchin , nicotinamide , phosphodiesterase , embryo , caffeine , chemistry , andrology , biology , embryogenesis , enzyme , biochemistry , endocrinology , microbiology and biotechnology , gastrulation , medicine
Treatment of sea urchin embryos with cAMP phosphodiesterase (PDE)‐inhibitors such as caffeine (4×10 −3 M), theophylline (8×10 −3 M), or nicotinamide (10 −2 M), at the morula stage for only a couple of hours, yields vegetalized larvae. Most of the embryos treated with these reagents before the morula stage develop to blastulae filled with mcsenchyme‐like cells. Almost all embryos at the blastula stage develop normally even if they are treated with a PDE‐inhibitor for a considerable period. The rate of 3 H‐valine incorporation into protein in the morulae is reduced by caffeine and theophylline, but does not decrease in the presence of nicotinamide. Actinomycin D cancels the vegetalizing effect of PDE‐inhibitors on the morulae.