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SITE OF 1‐METHYLADENINE RECEPTORS IN THE MATURATION OF STARFISH OOCYTES
Author(s) -
SHIDA HISATO,
HIRAI SETSURO
Publication year - 1978
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1978.00205.x
Subject(s) - starfish , germinal vesicle , oocyte , vitelline membrane , chemistry , vesicle , anatomy , biology , microbiology and biotechnology , membrane , biochemistry , embryo , ecology
Maturation of vitelline coat‐free (VCF) oocytes of the starfish, Asterina pectinifera , was studied. When the oocytes, the vitelline coats of which were elevated by adding the ionophorc A‐23187, were forced through two sheets of copper mesh, the vitelline coats were completely removed from the oocytes. Although some of the VCF oocytes underwent germinal vesicle breakdown following this mechanical treatment, most of them retained the normal germinal vesicles. These VCF immature oocytes underwent breakdown of germinal vesicles after addition of 1‐methyladenine (1‐MA). Dose‐response curves of VCF oocytes to 1‐MA were similar to those of normal oocytes. These results indicate that 1‐MA reacts with the plasma membrane and that the presence of the vitelline coat is not prerequisite for inducing oocyte maturation.