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MORPHOLOGICAL CHANGES OF CULTURED MYOCARDIAL CELLS DUE TO CHANGE IN EXTRACELLULAR CALCIUM ION CONCENTRATION
Author(s) -
GOSHIMA KIYOTA,
YAMANAKA HACHIRO,
EGUCHI GORO,
YOSHINO SHIGEO
Publication year - 1978
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1978.00191.x
Subject(s) - extracellular , incubation , calcium , verapamil , extracellular fluid , chemistry , biophysics , biology , medicine , biochemistry , organic chemistry
Increase in the extracellular Ca 2+ concentration from low (≤ 10 −7 M) to normal (10 −3 M) caused morphological changes of cultured myocardial cells obtained from fetal mouse heart. The extracellular Na + and K + concentrations of the normal medium (10 −3 M Ca 2+ ) did not significantly affect the genesis of these morphological changes. Like Ca 2+ , Ba 2+ and Sr 2+ , but not Mg 2+ , Co 2+ or Ni 2+ , could induce morphological changes. Increase in the extracellular Ca 2+ concentration from 10 −8 M to 10 −3 M also caused excess uptake of 45 Ca 2+ by cultured myocardial cells. B–16CW 1 cells, which did not show these morphological changes, did not take up excess 45 Ca 2+ on this treatment. Treatments, such as addition of verapamil or incubation at pH 6.3, which reduced the genesis of morphological changes, reduced the rate of 45 Ca 2+ uptake by myocardial cells. These facts show that the morphological changes of myocardial cells induced by increasing the extracellular Ca 2+ concentration from low to normal are due to excess uptake of Ca 2+ by the myocardial cells. The morphological changes of cultured myocardial cells induced by increasing the extracellular Ca 2+ concentration from low to normal were reversed on further incubation of the cells in medium with or without Ca 2+ .