Premium
MOUSE MUSCLE CELL DIFFERENTIATION IN CLONAL CULTURE
Author(s) -
NAKAMURA HARUKAZU,
FUJISAWA HAJIME,
WATANABE KENJI,
KUBO SEIICHI,
IBATA YASUHIKO
Publication year - 1978
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1978.00049.x
Subject(s) - myogenesis , myocyte , biology , cell culture , andrology , microbiology and biotechnology , fetus , tissue culture , anatomy , genetics , medicine , in vitro , pregnancy
Clonal culture of muscle cells from thigh muscles of the term fetus of the mouse was undertaken. Myoblasts were spherical or spindle‐shaped, and proliferated exponentially until day 4 in culture. The generation time of the muscle cells from 2 to 4 days' culture was 9.1 to 13.4 hr. The fusion of myoblasts began on day 4 in culture; many myotubes had been formed by day 6 and spontaneous contraction was observed on day 7. Clonal efficiency was 30%, and the proportion of muscle colonies in all the colonies was 72%.