Premium
EFFECTS OF THYROXINE AND PROLACTIN ON THE RATES OF PROTEIN SYNTHESIS IN THE THIGH BONES OF THE TADPOLE OF RANA CATESBEIANA
Author(s) -
YAMAGUCHI KEIICHIRO,
YASUMASU IKUO
Publication year - 1977
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1977.00161.x
Subject(s) - medicine , endocrinology , prolactin , tadpole (physics) , rana , proline , metamorphosis , leucine , thigh , biology , larva , amino acid , anatomy , biochemistry , hormone , ecology , physics , particle physics
In thigh bones isolated from a Rana catesbeiana tadpole which has been kept in a 5 × 10 −8 M thyroxine solution for several days, the rate of 14 C‐leucine incorporation into protein becomes higher than that in the thigh bones of control animals. Intraperitoneal injection of prolactin also results in an increase in the rate of 14 C‐leucine incorporation into protein in the thigh bones at a rate very similar to that in thyroxine‐treated animals. In the thigh bones of the thyroxine‐treated tadpoles, the rate of 14 C‐proline incorporation into protein is markedly higher than that of control animals. Prolactin treatment of the tadpoles also causes an increase in the rate of 14 C‐proline incorporation, but the rate is lower than that found in thyroxine‐treated animals. The injection of prolactin into thyroxine‐treated tadpoles fails to cause further increase in the rates of incorporation of these amino acids into protein. In the thigh bones of tadpoles at the climax of metamorphosis, prolactin injection does not cause any increase in the rates of 14 C‐labeled proline and leucine incorporation, whereas both rates become slightly higher in the thigh bones of thyroxine‐treated tadpoles at this stage. The thigh bones probably become insensitive to prolactin when they are exposed to thyroxine.