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INHIBITION BY BACTERIA OF PSEUDOPLASMODIUM FORMATION OF DICTYOSTELIUM DISCOIDEUM
Author(s) -
ISHIKAWA A.,
TAKAGI M.,
TATEISHI T.,
OHMORI H.
Publication year - 1977
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1977.00077.x
Subject(s) - dictyostelium discoideum , mutant , bacteria , mycetozoa , microbiology and biotechnology , chemistry , slime mold , biochemistry , biology , biophysics , genetics , gene
A mutant of Dictyostelium discoideum was isolated, the development of which was normal at 18.5°C but stopped at the aggregate stage when cultured with bacterial associate at 26°C. Probably the bacteria release a factor inhibitory to pseudoplasmodium formation, the inactivation of the inhibitor being temperature‐sensitive in this mutant. This bacterial factor is different from the one reported by W eber and R aper (16), judging from the synergism shown by these two types of mutants. The temperature‐shift experiment gave the result that the inhibited developmental step was at about the time of initiation of tip formation. However, once a tip was formed under the permissive conditions, the re‐formation of a new tip after removal of the original was not inhibited under the nonpermissive conditions. These results indicate that the bacterial inhibitor acts just before tip formation.