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POLYSOMES OF THE SEA URCHIN EMBRYO: NATURE OF SO‐CALLED “CYCLIC AMINO ACID INCORPORATION” AND CONDITIONS TO PREPARE CELL‐FREE SYSTEMS FOR PROTEIN SYNTHESIS
Author(s) -
HIRAMA MINORU N.,
MANO YOSHITAKE
Publication year - 1976
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1976.00363.x
Subject(s) - polysome , gtp' , amino acid , protein biosynthesis , cell free system , biochemistry , ribosome , chemistry , sea urchin , cell , reagent , embryo , cell free protein synthesis , biology , microbiology and biotechnology , organic chemistry , rna , in vitro , enzyme , gene
Cyclic formation of active polysomes in a cell‐free system, as reported previously, could not be demonstrated under the conditions reported. However, cyclic fluctuation was observed in incorporation of amino acids into the hot TCA‐insoluble fraction. Failure to demonstrate polysome formation seemed to be due to the lack of GTP and to the necessity to protect SH groups during preparation of the system and its assay. Addition of exogenous GTP and ME effectively restored polysomal activity in the cell‐free system. The previously reported cyclic incorporation of amino acids into the hot TCA‐insoluble fraction in cell‐free systems in the absence of these reagents might be due to cyclic variation in some partial reactions or some phenomenon other than protein synthesis.