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CONCANAVALIN A‐INDUCED AGGLUTINATION AND BINDING OF CON A TO THE DIFFERENTIATING CELLS OF DICTYOSTELIUM DISCOIDEUM
Author(s) -
KAWAI SHINJIRO,
TAKEUCHI IKUO
Publication year - 1976
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1976.00311.x
Subject(s) - dictyostelium discoideum , pronase , concanavalin a , agglutination (biology) , slug , endocytosis , cell , biology , lectin , microbiology and biotechnology , trypsin , chemistry , biochemistry , immunology , in vitro , antibody , gene , enzyme , cancer research
Changes in agglutinability of Dictyostelium discoideum cells with Concanavalin A (Con A) during the course of development were investigated. The agglutinability of the cells was assayed under conditions where no spontaneous cell agglutination occurred. It was found that there was a progressive decrease in Con A‐induced agglutinability during development: a decrease to half from exponentially growing cells to preaggregation cells, and to sixth in disaggregated slug cells. Pronase‐BAL treatment of preaggregation cells did not enhance their agglutinability with Con A. The amounts of sites available for binding Con A were determined with preaggregation and slug cells. Cells were incubated at 4°C and in the presence of NaN 3 to avoid possible endocytosis of Con A. No significant differences in numbers of Con A‐binding sites per unit area of cell surface was detected among preaggregation cells, those treated with pronase and BAL and cells disaggregated from slugs by similar treatment. It was thus concluded that the decrease in Con A‐induced agglutinability during development is not attributable to changes in the numbers of Con A‐binding sites.