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PRIMARY CULTURES OF THE CELLS FROM OVARIES OF THE CABBAGE ARMYWORM, MAMESTRA BRASSICAE L. (Lepidoptera: Noctuidae)
Author(s) -
MITSUHASHI JUN
Publication year - 1976
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1976.00163.x
Subject(s) - biology , lepidoptera genitalia , noctuidae , explant culture , fibroblast , diapause , microbiology and biotechnology , botany , cell culture , larva , in vitro , genetics
Ovaries from post‐diapause pupae of the cabbage armyworm, Mamestra brassicae , were cultured. Three types of cells migrated from the explants. Epithelial‐like cells formed confluent monolayers, and fibroblast‐like cells formed cellular networks. Isolated cells were distributed around the explants. Epithelial‐like cells and isolated cells were common, and fibroblast‐like cells were rather rare. The migrated cells survived for more than 6 months, although mitoses were rarely observed.