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MICROINJECTION OF COLCHICINE INTO SEA URCHIN EGGS
Author(s) -
HAMAGUCHI YUKIHISA
Publication year - 1975
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1975.00111.x
Subject(s) - cleavage (geology) , colchicine , microinjection , blastomere , sea urchin , anaphase , microbiology and biotechnology , mitosis , chemistry , biology , embryo , cell , biochemistry , embryogenesis , genetics , cell cycle , paleontology , fracture (geology)
Inhibition of cleavage by colchicine was examined by microinjecting colchicine solution into one of the blastomeres of a sea urchin egg at the two‐cell stage. Cleavage was inhibited if the microinjection was made before a critical point prior to the cleavage, whereas cleavage occurred in spite of the destruction of the mitotic apparatus if the microinjection was made after the critical point. The critical point was 10 min before the mid‐stage of the cleavage in Clypeaster japonicus and 8 min before the mid‐stage in Temnopleurus toreumaticus at 20 ± 1°C, corresponding to the beginning of anaphase. The threshold for the cleavage inhibition of colchicine was estimated to be 3 × 10 −5 M to 3 × 10 −6 M in final concentration in the cell.