Premium
THE SYNTHESIS OF 5S RIBOSOMAL RNA DURING POLLEN DEVELOPMENT
Author(s) -
PEDDADA LORRAINE B.,
MASCARENHAS JOSEPH P.
Publication year - 1975
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1975.00001.x
Subject(s) - microspore , ribosomal rna , rna , 5s ribosomal rna , pollen , biology , 18s ribosomal rna , period (music) , 23s ribosomal rna , botany , biochemistry , ribosome , stamen , gene , physics , acoustics
Tradescantia paludosa 5S ribosomal RNA (rRNA) has been characterized with respect to its base composition and relative electrophoretic mobility in comparison with that of E. coli . The period of 5S rRNA synthesis during pollen grain development was determined by pulse labeling the RNA synthesized during a 24 hr period of development with 32 P and then chasing in cold medium until pollen maturity. The period of highest 5S rRNA synthesis was found to occur prior to microspore mitosis. During and following mitosis over a period of 4 days there was a sharp decrease in the amount of 5S RNA synthesized and during the last 48 hr of pollen maturation, no 5S rRNA was synthesized.