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EFFECTS OF METHIONINE AND S ‐ADENOSYLMETHIONINE ON PRODUCTION OF 1‐METHYLADENINE IN STARFISH FOLLICLE CELLS
Author(s) -
SHIRAI HIROKO
Publication year - 1973
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1973.00307.x
Subject(s) - incubation , methionine , starfish , follicle , biology , incubation period , biochemistry , oocyte , chemistry , endocrinology , microbiology and biotechnology , embryo , amino acid , ecology
Incubation of follicle cells of the starfish ( Asterias amurensis ) with a gonad‐stimulating hormonal peptide (GSS) leads to the production of 1‐methyladenine. the trigger of oocyte maturation. Addition of L‐methionine to the incubation medium promotes the production of 1‐methyladenine. This study shows that S ‐adenosylmethionine also enhances the production of 1‐methyladenine in such an incubation mixture. However, in the absence of GSS, addition of S ‐adenosylmethionine failed to produce an appreciable amount of 1‐methyladenine. When an homogenate of isolated follicle cells was incubated, a certain amount of 1‐methyladenine was produced, whether or not GSS was present. Addition of S ‐adenosylmethionine to the incubation mixture of follicle homogenate enhanced the production of 1‐methyladenine. Although addition of adenosine triphosphate (ATP) to such an incubation mixture had little effect in producing 1‐methyladenine, it exerted a promoting effect on 1‐methyladenine production when S ‐adenosyl‐methionine was present. These results suggest that methionine, through its active form, S ‐adenosylmethionine, acts as a donor of methyl group in the formation of 1‐methyladenine.