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IN VITRO DIFFERENTIATION OF CHICK EMBRYONIC LENS EPITHELIAL CELLS INTO FIBER CELLS
Author(s) -
WATANABE HIROMITSU,
KAWAKAMI IZUMI
Publication year - 1973
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1973.00101.x
Subject(s) - embryonic stem cell , microbiology and biotechnology , in vitro , lens (geology) , lens fiber , biology , cellular differentiation , gene , genetics , paleontology , nucleus
The processes of fiber‐cell formation in the lens epithelium of 9‐day‐old chick embryo in vitro were studied. Mitotic activity was enhanced during the first 12 hr, but with a drop at the 4th hour of cultivation. After the 24th hour, when the cells began to elongate, almost no mitotic figures or incorporation of 3 H‐thymidine into the nuclei were observed. α‐ and δ‐crystallin were contained in and synthesized by the newly isolated lens epithelium. The content and syntheses had diminished by the 12th hour. In the earlier phase of cultivation, both fiber cell formation and crystallin synthesis were suppressed by treatment with Actinomycin D, but after the 12th hour they were resistant to the antibiotic. The correlation between cell division and fiber‐cell differentiation in the lens epithelium in vitro is discussed and compared with that reported in Wolffian lens regeneration and in developing bovine lens.