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ONTOGENY OF CHICKEN HEMOGLOBIN. I. ELECTROPHORETIC STUDY OF THE HETEROGENEITY OF HEMOGLOBIN IN DEVELOPMENT
Author(s) -
SHIMIZU KOJI
Publication year - 1972
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1972.00043.x
Subject(s) - embryo , fetus , hemoglobin , incubation , embryogenesis , biology , embryonic stem cell , ontogeny , andrology , fetal hemoglobin , gel electrophoresis , starch , microbiology and biotechnology , biochemistry , chemistry , genetics , pregnancy , medicine , gene
Separation of different molecular species of hemoglobin from developing chickens by starch gel electrophoresis has revealed the appearance of early embryonic (embryonic), late embryonic (fetal) and adult hemoglobin (Hb) type during development. In 5‐day embryos, there are 3 or 4 forms of embryonic Hb type. They begin to decrease in 6‐day embryos and cannot be detected in embryos after 10 days of incubation. In 6‐day embryos, two forms of adult Hb type appear, and one of them, which is a major form in adults, becomes t o be a major one in 7‐day embryos. One or two forms of fetal Hb type first appear in 10‐day embryos and are still present in 5‐day posthatching chickens. Ultracentrifugation of carbonmonoxyhemoglobins from embryos at early and at later stages (fetuses), from newly hatched and from adult chickens has shown that they have a single monodisperse peak. Some heterogeneity, however, has been detected after starch gel electrophoresis, probably owing to aggregation or polymerization. Subunit analysis of embryonic, fetal and adult Hb type by starch gel electrophoresis in formate buffer at pH 1.9 has indicated that embryonic Hb type contains total 5 subunits, C, D, E, F and G; fetal Hb type, total 2, A and H; and adult Hb type, total 3, B, F and H.