Premium
BIOCHEMICAL CHANGES IN YEAST DURING SPORULATION. II. ACETATE METABOLISM
Author(s) -
MIYAKE SETSUKO,
SANDO NOBUNDO,
SATO SITIRO
Publication year - 1970
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1971.285_n.x
Subject(s) - glyoxylate cycle , isocitrate lyase , biochemistry , strain (injury) , isocitrate dehydrogenase , citric acid cycle , metabolism , dehydrogenase , biology , auxotrophy , enzyme , saccharomyces cerevisiae , yeast , aconitase , ploidy , succinate dehydrogenase , escherichia coli , gene , anatomy
A bstract Acetate metabolism was studied with Saccharomyces cerevisiae diploid strain G2‐2 in sporulating culture, asporogenic diploid strains 3c × a and 3c × 3a, and respiratory deficient haploid strain 3c (asporogenic). Acetate in a sporulating medium was utilized by sporogenic and asporogenic diploid yeasts linearly with time. Activities of aconitase, NADP‐linked isocitrate dehydrogenase, and succinate dehydrogenase initially increased in the cell‐free homogenate of either strain. Activity of glucose‐6‐phosphate dehydrogenase decreased. Isocitrate lyase activity increased remarkably in the sporogenic strain but not in the asporogenic strain. The rate of production of 14 CO 2 from 14 C‐1‐acetate was accelerated more than from 14 C‐2‐acetate in intact cells of the sporogenic strain during sporulating culture. Fractionation of radioactive cell substances showed remarkable lipid synthesis. Accumulation and reutilization of cold acid‐soluble precursor substances occurred during sporogenesis. The role of glyoxylate and tricarboxylate cycle enzymes in sporulation is discussed.