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CELLULAR ANALYSIS ON LOCALIZATION OF LENS FORMING POTENCY IN THE NEWT IRIS EPITHELIUM *, **
Author(s) -
EGUCHI GORO,
SHINGAI RYUZO
Publication year - 1971
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1971.00337.x
Subject(s) - iris (biosensor) , lens (geology) , epithelium , potency , anatomy , microbiology and biotechnology , biology , computer science , artificial intelligence , genetics , paleontology , biometrics , in vitro
The localization of a lens forming potency in the iris epithelium was studied by autoradiographic analysis of the distribution of 3 H‐thymidine labelled cells to be participated in lens regeneration in newts. DNA synthesis started from the dorsal portion of the iris epithelium around 4 days after lentectomy. 5 days after lentectomy, a large number of labelled cells were mostly found in the dorsal sector, showing strong contrast to the ventral and lateral sectors of iris, which contained a few labelled cells. The labelled index (the number of labelled cells/the number of cells in the definite pigmented area of the iris epithelium) of the dorsal sector attained the highest value, 29.7 ± 2.35, on day 7 after lentectomy, and dropped temporarily. This was followed by the second peak on day 12. The dorso‐ventral ratio of the labelled index reached to the highest value, 6.87 ± 0.67, on day 5. This ratio decreased rapidly after the completion of a lens rudiment, and it became about 1. In “chase” experiments by diluting the radio‐isotope with excess cold thymidine, it was obviously shown that most of the cells labelled with the radio‐isotope and distributed in the dorsal marginal iris 5 days after lentectomy participated in the formation of a lens regenerate during the period of chasing. From these results, the following conclusion was drawn. The iris epithelium consists of at least 2 different cell populations; one is capable of transformation into lens cells and is distributed mostly in the dorsal portion of the iris epithelium, while the other has no potency for transformation and is able to grow to compensate a loss of the dorsal marginal cells which transformed into lens cells during the process of lens regeneration.