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ULTRASTRUCTURE OF THE DIFFERENTIATED CELL COLONY DERIVED FROM A SINGLY ISOLATED CHONDROCYTE IN IN VITRO CULTURE
Author(s) -
EGUCHI GORD,
OKADA T. S.
Publication year - 1970
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1971.00297.x
Subject(s) - ultrastructure , endoplasmic reticulum , vacuole , lumen (anatomy) , microbiology and biotechnology , chondrocyte , biology , anatomy , chemistry , cartilage , cytoplasm
A bstract The ultrastructure of differentiated colonies developed in vitro from singly isolated chondrocytes of the sterna of chicken embryos were studied by an improved method for cutting ultra‐thin sections for electron microscopy in a plane normal to the plastic substrate. The differentiated colony consisted of a central refractile portion with a metachromatic matrix and a peripheral monolayered ring. Electron microscopic observation of thin sections showed a large central lumen which seems to be filled with cartilaginous matrix and collagen. Its roof is a thin layer of cells, in which the cells come together rather compactly, while the floor of the lumen consists of a monolayer, in which conical cells adhering to the substrate were loosely distributed. Most cells in the central portion are rich in granular endoplasmic reticulum with prominent saccular cisternae and large vacuoles presumably derived from Golgi complexes. The cells adhering to the substrate are actually in contact with it at only a few points across a gap of 40–200 A. The rest of the cell surface is free of the substrate.