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BIOCHEMICAL CHANGES IN YEAST DURING SPORULATION. I. FATE OF NUCLEIC ACIDS AND RELATED COMPOUNDS
Author(s) -
SANDO NOBUNDO,
MIYAKE SETSUKO
Publication year - 1970
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1971.00273.x
Subject(s) - nucleic acid , biology , spore , meiosis , uracil , sporogenesis , dna , yeast , saccharomyces cerevisiae , protein biosynthesis , rna , phase (matter) , ploidy , biochemistry , dna synthesis , dna replication , microbiology and biotechnology , chemistry , gene , organic chemistry
A bstract Changes in nuclear figures and in activities of nucleic acid and protein syntheses were observed mainly on Saccharomyces cerevisiae G2‐2 during sporogenesis. Patterns of DNA synthesis and of meiosis show that the sporogenic process in yeast was divided into an induction phase (I‐phase), a DNA‐synthesizing phase (S‐phase) and a maturation phase (M‐phase). Meiotic figures appeared most frequently at the end of the S‐phase at approximately 12 hr in sporulation culture. In M‐phase visible spores formed. The amount of protein increased in the initial 7 hr culture of 1‐phase, then decreased in the S‐ and M‐phases. But in sporulation culture of the asporogenic diploid strain 3c × a, protein did not decrease. RNA increased within 3 hr of the I‐phase then stopped increasing. DNA synthesis occurred critically during S‐phase, i.e. between 7 and 12 hr. and was somewhat resumed during the later part of M‐phase. Oligodeoxyri‐bonucleotide content decreased in the I‐ and M‐phases and increased temporarily. Deoxyribosides decreased linearly during the sporogenic processes. Based on these results and results of experiments estimating the incorporation of 14 C‐uracil into nucleic acid and 14 C‐amino acid mixture into protein fractions, the roles of nucleic acid synthesis activities in meiosis and in sporulation are discussed.