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ACCUMULATION OF 4‐ O ‐β‐D‐GLUCOSIDE OF PROTOCATECHUIC ACID IN THE LEFT COLLETERIAL GLAND OF THE COCKROACH, PERIPLANETA AMERICANA . * I. PROPERTIES OF GLUCOSIDE SYNTHETASE.
Author(s) -
TAKAHASHI SUSUMU Y.
Publication year - 1971
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1971.00131.x
Subject(s) - protocatechuic acid , cockroach , glucoside , periplaneta , chemistry , enzyme , biochemistry , phenols , phenol , biology , organic chemistry , antioxidant , medicine , ecology , alternative medicine , pathology
As a preliminary to the study of the mode of action of juvenile hormone using the left colleterial gland of the cockroach, properties of the glucoside synthetase of protocatechuic acid were studied. For this purpose, a convenient assay method of phenolic glucoside synthetase (UDPG‐phenol glucosyl transferase) was devised. The method is based on the determination of labeled protocatechuic acid glucoside which is synthesized from uridine diphosphate 14 C‐glucose and protocatechuic acid. The optimal pH for the glucoside synthetase reaction is 6.5. The enzyme activity is localized in a particulate fraction of fat body tissues and is partly found in the soluble fraction, presumably liberated during the course of centrifugation. In the female cockroach, the enzyme activity is distributed mainly in the fat body. Chemical analyses revealed that the reaction product is 4‐ O ‐β‐D‐glucoside of protocatechuic acid. The homogenate of fat body tissues can synthesize glucosides from UDPG and various phenols, such as O ‐aminophenol, p ‐nitrophenol and hydroquinone, as well as natural O ‐diphenols.