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SEPARATION OF TWO FACTORS AFFECTING THE AGGREGATION KINETICS FROM THE CONDITIONED MEDIUM
Author(s) -
Takahashi K.,
Okada T. S.
Publication year - 1971
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1971.00015.x
Subject(s) - sephadex , kinetics , flocculation , plating efficiency , fraction (chemistry) , chemistry , growth factor , biophysics , cell , chromatography , biology , biochemistry , receptor , enzyme , physics , organic chemistry , quantum mechanics
Two effective factors contained in medium “conditioned” with a large number of cultured cells were separated through Sephadex G‐25 gel, by assaying the plating efficiency and growth rate of chicken embryonic cells in secondary culture. The first factor, which is contained in the heavy molecular weight fraction α, can promote plating efficiency of the inoculated cells at low density. The second is contained in the light molecular weight fraction β, and promotes the growth rate of sparsely cultured cells. The effects of these factors upon the aggregation kinetics of singly isolated cells were studied. The factor in the fraction α promotes aggregation to result in fast flocculation. The factor in the fraction β, while not effective by itself, affects the aggregation of the cells treated with the factor in α. In the co‐presence of both α and β, a “lag” was found after the initial fast flocculation. The possible mechanisms of the interaction of these two factors on the cell surface are discussed.