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EFFECTS OF BENZAMIDE ON EMBRYONIC DEVELOPMENT OF THE HOUSEFLY
Author(s) -
BHASKARAN G.,
RAMAKRISHNAN V.,
ADEESAN C.
Publication year - 1970
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1970.00265.x
Subject(s) - blastema , housefly , biology , benzamide , microbiology and biotechnology , embryogenesis , embryo , rna , embryonic stem cell , cleavage (geology) , regeneration (biology) , chemistry , biochemistry , botany , musca , larva , gene , paleontology , organic chemistry , fracture (geology)
A bstract This paper deals with the effects of benzamide, a chromosomal RNA inhibitor, on embryonic development of the housefly Musca domestica nebulo . Eggs exposed to benzamide immediately after oviposition continued to develop until the blastema stage, but further development was arrested. Formation of cell boundaries and nucleoli failed to occur and the nuclei at the posterior pole did not differentiate into pole cells. This suggests that synthesis of new RNA is needed for development beyond the blastema stage. Treatment of eggs at different stages of development showed that as development progressed embryos became less sensitive to the drug. Introduction of benzamide into eggs during the pre‐blastema period caused irreversible arrest of development, whereas, treatment during post‐blastema stages resulted in reversible inhibition of development. The cortical cytoplasm thus appears to induce a significant change in the cleavage nuclei, which alters their sensitivity to the drug.