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EFFECTS OF DNP‐ AND NaN 3 ‐PRETREATMENT ON CLEAVAGE OF THE SEA URCHIN EGG *
Author(s) -
KOJIMA MANABU KUNO
Publication year - 1970
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1970.00255.x
Subject(s) - hemicentrotus , sea urchin , parthenogenesis , cleavage (geology) , chemistry , reproductive cycle , biology , fishery , microbiology and biotechnology , embryo , ecology , reproduction , paleontology , fracture (geology)
A bstract Unfertilized eggs of the sea urchin, Hemicentrotus pulcherrimus were treated with DNP‐sea water (M/500 ‐ M/2,000) or with NaN 3 ‐sea water (M/50 ‐ M/800) for 15 – 90 minutes, and 2 hours later they were inseminated. Such a treatment induced acceleration of the first cleavage, and the eggs had a tendency to divide directly into 4 cells in the first cleavage cycle. In another series of experiments, unfertilized eggs were developed parthenogenetically by L oeb 's double‐treatment after the above‐mentioned treatment. It was found that these eggs divided earlier than the controls and that they developed into swimming blastulae in higher percentages. From the results described in the present and other papers it is suggested that DNP and NaN 3 have effects to activate eggs parthenogenetically and also to induce division of the centrioles, closely relating to the cleavage.