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CHANGES IN ELECTRONEGATIVITY OF THE CELL SURFACE DURING THE DEVELOPMENT OF THE CELLULAR SLIME MOLD, DICTYOSTELIUM DISCOIDEUM *
Author(s) -
YABUNO KYÔZÔ
Publication year - 1970
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1970.00229.x
Subject(s) - dictyostelium discoideum , slime mold , interphase , pronase , electrophoresis , chemistry , proteolytic enzymes , enzyme , biochemistry , biophysics , biology , microbiology and biotechnology , trypsin , gene
A bstract A microelectrophoretic method was applied to determine electrophoretic mobility of the amoebae of the cellular slime molds at various stages of development. The vegetative amoebae were negatively charged and their electrophoretic mobility remained unchanged as long as they fed. After the cessation of feeding mobility of the amoebae decreased gradually in the interphase. Proteolytic enzymes and EDTA had no effect on mobility of the amoebae right after finishing feeding. On the contrary, mobility of the amoebae in the middle interphase was increased by treatment with proteolytic enzymes, lipase, and EDTA. EDTA and pronase showed an additive effect on the increase in mobility, and the increased mobility was almost the same as that of the vegetative a‐moebae. These results indicate that the decrease in mobility during the interphase is due to the accumulation of a substance(s) (probably lipoprotein) on the cell surface. The cells disaggregated from the migrating slugs showed lower mobility than the cells obtained from the aggregation centers. The latter had still lower mobility than the interphase amoebae. These changes in electrophoretic mobility were discussed in relation to corresponding changes in adhesiveness.