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EFFECTS OF CHEMICAL DISRUPTION ON THE BIOLOGICAL ACTIVITIES OF SEA URCHIN EGG JELLY 1
Author(s) -
ISHIHARA K.,
DAN J. C.
Publication year - 1970
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1970.00179.x
Subject(s) - sodium periodate , sea urchin , biochemistry , chemistry , agglutination (biology) , periodate , sialic acid , acrosome reaction , hemicentrotus , pronase , fucose , sperm , trypsin , enzyme , glycoprotein , chromatography , biology , organic chemistry , botany , microbiology and biotechnology , genetics , antigen , in vitro
A bstract In an attempt to relate the biological activities of sea urchin egg jelly to the structural characteristics of the acid glycoprotein molecule, the jelly was oxidized with H 2 O 2 and sodium periodate, and digested with trypsin and pronase. The non‐dialyzable products of H 2 O 2 and periodate oxidation, and a fucose‐rich fraction isolated from enzyme‐digested jelly by column chromatography, were tested for their capacity to induce sperm agglutination and acrosome reaction in Hemicentrotus pulcherrimus . It was found that a degree of enzyme digestion sufficient to remove about 80% of the amino acids reduced, but failed to eliminate, the capacity of the jelly to elicit agglutination and acrosomal reaction. Mild oxidation with H 2 O 2 suppressed sperm agglutination, but more drastic treatment was required to destroy the capacity of the jelly to induce the acrosome reaction. The loss of both these biological activities after periodate oxidation was found to parallel the release of sialic acid.