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CHANGES IN THE CAPACITY FOR CLONAL GROWTH AND DIFFERENTIATION IN VITRO OF THE VERTEBRAL CARTILAGE CELLS WITH EMBRYONIC DEVELOPMENT. 1. CULTURE IN THE STANDARD MEDIUM
Author(s) -
WATANABE KAZUO
Publication year - 1970
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1970.00079.x
Subject(s) - cartilage , embryo , in vitro , microbiology and biotechnology , biology , embryonic stem cell , cellular differentiation , anatomy , andrology , immunology , genetics , medicine , gene
A bstract When culturing the vertebral cartilage cells of the chick embryo, the youngest embryo from which the dissociated single cell of the cartilage gives rise to the differentiated cartilage colony (CMC) in the given conditions of the clonal cell culture is at st.26. At this stage, percentage of such cells to make CMC in vitro is less than 0.1% of the inoculated single cells. With development, percentage of such “stabilized” cells increases, finally reaching to 35% at st.40. In embryos from st.29 to st.36, many of the cultured single cells give rise to the regressing colonies (RGC) which show some proliferation at the early stage in vitro , but in due time cease to proliferate and regress.