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GROWTH OF THE CHICKEN EMBRYONIC LENS TRANSPLANTED ONTO THE CHORIO‐ALLANTOIC MEMBRANE
Author(s) -
TAKEICHI MASATOSHI
Publication year - 1970
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1970.00021.x
Subject(s) - biology , lens (geology) , retina , transplantation , incubation , microbiology and biotechnology , embryonic stem cell , embryo , lens fiber , anatomy , epithelium , medicine , biochemistry , neuroscience , genetics , paleontology , gene
A bstract The influence of neural retina on the growth of chicken embryonic lens was studied by comparing the growth pattern of the lens transplanted onto chorio‐allantoic membrane (CAM) with that of the normal lens. The lens from 6‐day embryo, transplanted onto CAM after labeled with 3 H‐thymidine, continued to grow in the absence of neural retina at least for 12 days of incubation, although its growth rate was reduced. In the transplanted lens, no 3 H‐labeled epithelial cell differentiated into fiber at least for 2 days of incubation and 3 H‐labeled nuclei first appeared in the fiber cells on the fourth day of incubation, while, in the normal lens of 6‐day embryo labeled with 3 H‐thymidine in situ , 3 H‐labeled epithelial cells differentiated into fibers within 24 hours. On the other hand, the fiber cells differentiated before transplantation maintained the nearly normal growth rate on CAM. The neural retina transplanted onto CAM together with lens induced the new fibers from the lens epithelium. These observations suggest that the neural retina initiates and promotes the fiber differentiation in the chicken lens, but its continued influence is not always necessary for the successive differentiation of epithelial cell into fiber and especially for the growth of the differentiated fiber cells.